The study describes the use of selective high PI protein extraction and enrichment of human serum albumin (HSA) modified gold nanoparticles (HSA-AuNPs), lysozyme (LYZ) prior to analysis by capillary electrophoresis (CE)with UV detection. HSA gold nanoparticles can be extracted from a complex matrix LYZ HSA the caprock not only stable gold nanoparticles in high salt environment, but also showed a strong electrostatic attraction LYZ neutral pH conditions. LYZ and other high isoelectric point (pI) of the efficient separation of the protein has been successfully achieved by the cationic polyelectrolyte, poly (dimethyl diallyl ammonium chloride the) (PDDAC) filling, background electrolyte. Capture and HSA-AuNPs LYZ PDDAC filled CE can be directly used for analysis of the extracted LYZ releasing agent is not added to the extractor. Extraction efficiency depends on the gold nanoparticles of the solution pH and concentration of HSA. In the preferred extraction conditions, the signal-to-noise ratio of the detection limit of 3 LYZ of 8nm or less. HSA-AuNPs extraction and PDDAC of a combination of filling the CE has been the application of the analysis LYZ egg white, human milk, and the human tear. In addition, based on the extraction of the NP may be coupled to matrix-assisted desorption / ionization time-of-flight mass spectrometry and sodium dodecyl sulfate - polyacrylamide gel electrophoresis

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Recently, researchers from the University of Wisconsin, etc. found in the insect the key structural component of an enzyme that plays multiple roles, including the role of the synthesis of epidermis and melatonin ,and biogenic amines detoxification. And this enzyme is active in aedes aegypti, which is known as the disseminator of yellow fever and dengue fever..The study results were published in a recent journal PNAS.

Researcher Jianyong Li and his colleagues studied this enzyme – fragrant alkyl amine N-acetyl transferase (aaNAT). Using bioinformatics techniques to divided the insect sequence which is similar to aaNAT into three clusters, and then using crystallographic techniques, the team observed three clusters of enzymes in the crystal structures, and finally found that the biochemical active component of the protein from the two clusters enzymes. It is worth noting that the research team found a unique cluster of enzymes in the mosquito.

Next ,the researchers will further study the physiological function of this particular enzyme in mosquitoes.In short, the researchers' findings provides a basis for the biochemical function study of the mosquitoes specific enzymes,and provides ideas and guidance for the development of a potential mosquito-killing agent.

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German researchers found that a bacterial toxin can cause a protein inactivation within the cell, resulting in cell "suicide". This finding helps to study how to kill cancer cells. Related paper was published in "Journal of Biological Chemistry". Germany University of Freiburg, issued a press statement on August 1 that, university researchers found a kind of toxincalled "Tpel” in Clostridium perfringens, which can lead to gas gangrene. The toxin can cause sugar molecules attached to Ras proteins in the host cell to interfere with the natural functions of this protein. Pharmacologist Klaus, Aketorresfrom University of Freiburg told us that , Ras protein in human cells play an important role in stimulating cell growth, the toxin Tpel will change this protein to fail to promote cell growth, ultimately leading to cell death. It often occurs Ras protein mutation In human cancer cells, and this protein plays an important role in cancer formation.If target the variant Ras protein in the cells, and we can perhaps use such toxins lead to cancer cells "suicide." The researchers are now conducting further studies.

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