Antisense oligonucleotides (management) has been used for prostate cancer model for growth regulatory proteins, and at least one oligosaccharides (against BCL  has reached the clinical test. We've found that in LNCaP cell, mono - and double function management, the relative inhibitory expression, as compensation, the base class library 2 inhibition caspase 3 (apoptosis promoter) activities, and increase the expression of androgen receptor (AR) and p300 and il - co catalyst. In addition, the prostate specific membrane antigen (PSMA) and (may be its regulators) interferon (IFN) rise. 14 protein distributed between regulators of apoptosis, androgen control, differentiation antigen and autocrine mediated growth has been inspection. We put these findings including vascular endothelial growth factor (VEGF), promote angiogenesis, not through the compensation significant change, so do not need additional supervision for repression BCL 2 treatment is effective (such as caspase 3).

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Trichostatin (TSA) as a reason inhibitor can adjust many biological properties including the cell apoptosis and cell proliferation in all kinds of cancer cells. Here, we evaluate the effects of TSA in growth and death sheila cervical cancer cells and reactive oxygen species (ROS) and glutathione (GSH) content. Dose - and the time to observe somatostatin in hella cells and a IC50 about 20 nM 72 h. The agency also induced apoptosis cells die, as annexin v bovine serum albumin dyed cells, caspase 3 activation and the loss of mitochondrial membrane potential (MMP; ∆ ψ m). In addition, the government of the base class library 2 small interference RNA aggravate tsa induced hela cell death. All the testing caspase inhibitor greatly save some cell tsa induced hela cell death. TSA increased, O2 - level and inducing glutathione hella cell. Caspase inhibitor decreased obviously, O2 - level, GSH hella cell tsa treatment. In addition, n-acetyl cysteine (NAC; a known antioxidant) significantly prevent cell death and glutathione tsa treatment of hella cells, by reducing the O2 - level. In short, TSA cytostatic sheila through the BCL 2 mediated apoptosis closely related, O2,, glutathione levels.

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Secretion clusterin (sClu) is a kind of disease of protein, played an important role in protecting cells from tumor necrosis factor (TNF) α induced apoptosis. The purpose of this study was to survey the molecular mechanism of the influence sClu TNF induced apoptosis in breast cancer cells. The wild type of p53 expression MCF 7 cell line was designed out excessive performance sClu (MCF 7 / sClu), and mda - m - 231 cell line and mutant p53 and sClu son transfection is the silence of the small interference RNA (mda - m - 231 / sClu small interference RNA). Clusterin overexpression influence, in the cell apoptosis and sensitivity are two TNF α in vitro. Our results show that TNF treatment increase BCL 2 mRNA and protein content in breast cancer cells, which suggests that BCL 2 directly subject to nuclear factor - kappaB (NF - kappaB) in response to TNF α. The induction of the base class library 2 mediated p65 subelement of NF - kappaB. The silence of the small interference RNA mediated BCL 2 lead to increased significantly TNF induced apoptosis. The silence of the sClu in mda - m - 231 / sClu small interference RNA cell abolished TNF mediated NF - kappaB activation and BCL 2 overexpression, and present mda - m - 231 / sClu small interference RNA cell significantly more sensitive, TNF than parent mediated apoptosis. In addition, sClu expression in the MCF 7 / sClu cell mediated TNF NF - kappaB promotion activities and BCL 2 overexpression, and presents MCF 7 / Clu cell significant resistance to TNF α mediated apoptosis. Inhibition of NF - kappaB activity or p65 and BCL 2 expression reverse these effects. The results show that sClu awarded breast cancer cells apoptosis induced resistance of TNF α by NF - kappaB activation and BCL 2 overexpression.

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Invertebrate lysozyme (I - lyz or destabilase) exist in shrimp. The protein may have a function as a peptidoglycan break enzyme and as a peptide enzyme. Shrimp usually vibrio infection. , gram negative bacteria, as is known to all, c - lyz (similar to the chicken lysozyme) is active against these bacteria. In order to further understand renaturation of rules, we determine the sequences of genes and protein structure I - lyz modeling. In addition, I - lyz expression and c - lyz response lipopolysaccharide (LPS) was studied. Shrimp I - lyz gene is made up of two introns interrupt to standardize joint connection. Expression of shrimp I - lyz is transient cut injection followed by after LPS induced after 6 hours in hepatopancreas. By contrast, c - lyz is raised hepatopancreas 4 h post injection and trimmed in gill. L. vannamei I - lyz does not contain of lysozyme catalytic residues (glycohydrolase) is not isopeptidase activities; However, as is known to all, antibacterial activities not only rely on the enzyme activity of protein. Research invertebrates lysozyme will increase our understanding of the regulatory process defense mechanism.

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In order to use lysozyme as an antimicrobial agent in the brewing process, the hen protein lysozyme (LYZ) is in two different covalent immobilization micro size magnetic particle (toluene sulfonyl activation and carboxylated, TSA and CA, respectively). A cell suspension of Oenococcus oeni, a oenological strain to participate in brewing process, be used as LYZ substrate. At the same time, a research dynamics study the stability of a LYZ free and fixed in the McIlvane buffer in the pH value is 3.2, the lowest pH value in wine. Activities and dynamics parameter measuring free LYZ pH value is 3.2, below reported in the best pH value (4.5); However residual activity in the pH value 3.2 is enough interests for further fixed and applications in the wine. All the dynamics parameters two biological catalyst (LYZ - CA and LYZ - TSA) after fixed change, this may be due to the active site structure modification caused by covalent attachment support. At 25 ° C is free the half-life of calculation LYZ 39 h, but it increases to 280 and 134 h and LYZ - CA LYZ - TSA of respectively. The results show that the stability of the immobilized enzyme and improve, can use LYZ in wine application in its fixed form. In addition, LYZ - TSA seems to be the best biological catalyst for further application in wine.

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The study describes the use of selective high PI protein extraction and enrichment of human serum albumin (HSA) modified gold nanoparticles (HSA-AuNPs), lysozyme (LYZ) prior to analysis by capillary electrophoresis (CE)with UV detection. HSA gold nanoparticles can be extracted from a complex matrix LYZ HSA the caprock not only stable gold nanoparticles in high salt environment, but also showed a strong electrostatic attraction LYZ neutral pH conditions. LYZ and other high isoelectric point (pI) of the efficient separation of the protein has been successfully achieved by the cationic polyelectrolyte, poly (dimethyl diallyl ammonium chloride the) (PDDAC) filling, background electrolyte. Capture and HSA-AuNPs LYZ PDDAC filled CE can be directly used for analysis of the extracted LYZ releasing agent is not added to the extractor. Extraction efficiency depends on the gold nanoparticles of the solution pH and concentration of HSA. In the preferred extraction conditions, the signal-to-noise ratio of the detection limit of 3 LYZ of 8nm or less. HSA-AuNPs extraction and PDDAC of a combination of filling the CE has been the application of the analysis LYZ egg white, human milk, and the human tear. In addition, based on the extraction of the NP may be coupled to matrix-assisted desorption / ionization time-of-flight mass spectrometry and sodium dodecyl sulfate - polyacrylamide gel electrophoresis

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