Methyl mercury is an environmental pollutant, poisonous central nervous system; However, its toxic molecules mechanism is not clear. Methyl mercury increase several chemokine expression in cerebellar mice and methyl mercury. This study analyzed the potential of the methyl mercury induced chemokine expression mechanism use human 1321 n1 astrocytes, and display, methyl mercury increases in these cells CCL2 expression. The transcription factor nf-kappa B is involved in induced chemokine expression. Methyl mercury levels increase the nf-kappa B p65 subelement in the nuclei of 1321 n1 cell. Methyl mercury cause increase significantly reduce the CCL2 expression inhibition p65 expression by RNA interference. The results show that methyl mercury cause the nf-kappa B chemokine expression through the activation in human astrocytes

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Food allergy (FA) is frequent in 8% of children under 3 years old and about 2% of adults. Chemokine is involved in all kinds of allergies such as fa. The purpose of this study is to determine the CCL2, CCL5, CCL11 level in the fa cup. The study population cross-sectional study contains 63 patients suffering from the fa cup and 100 healthy controls. The concentration of the CCL2, CCL5, CCL11, IgE measurement using enzyme-linked immunosorbent assay (ELISA). Use Kathy Ι eosinophils number cell count + analyzer system model SCAREF system GmbH. Is considered to be an important difference, P < 0.05. The results show that the number of FA patients significantly increased than cycle without disease control peripheral eosinophil. The fa patients serum immunoglobulin e level is higher than the control. We also show that serum CCL2 and CCL11 was significant increase in the fa, but compared with patients who CCL5 not detectable control. The results of this study showed that two CCL11 CCL2, more elevated FA children with allergic reaction and urticaria than bronchial asthma and allergic dermatitis. These results also show that more level of increase and CCL11 CCL2 observation after consumption of milk and pistachios. Overall, the results suggest that serum CCL2 and CCL11 rise FA and these may be considered useful parameters in the diagnosis of the disorder. Still can design treatment on the basis of, through the application block chemokine expression antibody they overcome allergic patients with complications in the fa.

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Allele expression imbalance (AEI) is a kind of important genetic factor is the difference of the phenotypic traits, can genetic. The study can be used to find factors that regulate gene expression molecular mechanism, which is helpful to understand the bottom phenotypic change. Although it is generally accepted in many species, and we know that many genes show allele expression imbalance, this kind of phenomenon is not large scale study in cattle. Use pyrophosphoric acid sequencing technology method we analyzed a group of 29 cattle genes in order to find the favorable allele expression. The study investigated in three organization: liver, pituitary and kindey. From the research group of genes and their mineral wax (leptin), IGF2 (insulin-like growth factor 2), the CCL2 (chemokine carbon-carbon bond pattern ligand 2) display allelic expression is not balanced.

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Endocrine disorders that affect coagulation and fibrinolysis system and related the development of cardiovascular disease. Thrombin increase can activate fibrinolysis inhibitors (TAFI) is a link between coagulation and fibrinolysis system. The purpose of this study was to determine the effect of thyroid hormone excess and lack of in TAFI level and function. In TAFI hyperthyroxinemia was studied in healthy volunteers were randomly assigned to accept left-hand thyroid hormone or no drug treatment for 14 days in crossover design. In TAFI hypothyroidism was studied in a multi-center observational cohort study. Pumping blood therapy patients before, when euthyroidism newly diagnosed the hypothyroidism. Plasma clot dissolution time, activation TAFI (tower Philippines and wine) depend on the extended blood clot dissolved and TAFI level measurement. Thyroid hormone excess lead to hypofibrinolytic conditions and in an enhanced tower Philippines and alcohol dependence extended blood clot dissolved. The trend of decline TAFI observation plasma levels in healthy volunteers use left-hand thyroid hormone. Hypothyroidism cause hyperfibrinolysis and reduce the tower Philippines and alcohol dependence extended blood clot dissolved. In a word, the change of the tower Philippines and alcohol dependence extended blood clot dissolved in patients with thyroid disease may lead to a damaged hemostatic balance. In the disturbance hemostatic patients balance hyperthyroidism may make them easy to thrombosis, and the risk of bleeding may increase patient hypothyroidism.

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Alpha thrombin of serine protease is a kind of protein, can be used as the blood coagulation cascade. Thrombinprocoagulant itself is a differences, fibrinogen make fibrin clot, but thrombin - thrombomodulin (TM) complicated start anticoagulation path, through the crack protein c. TM fragment of only the fifth and sixth egf like domain (TM56) enough to bind thrombin, but there are also the fourth egf like domain (TM456) induced vital anticoagulant activity of thrombin. Crystallography of thrombin - TM456 complex and not found in the thrombin bring significant structural change, this shows that TM4 may only provide a scaffold best calibration of protein C for the cleavage, thrombin. However, all kinds of experimental data show that exist TM4 may affect the dynamic properties of the active site cycle. In the present work, we use two traditional and accelerate the molecular dynamics simulation method is used to study the dynamic characteristics of the structure, thrombin of thrombin: TM56, thrombin: TM456 in a wide range of time scales. Two different but related allosteric way, while determining mediation pro - and anticoagulant blood clotting enzyme activity. A allosteric way, at the same time, present in the thrombin: TM56 and blood coagulation enzyme: TM456, direct connection TM5 domain of thrombin active site. Other allosteric path, which only appears in the slow time scale exist TM4 domain, including prolong the network related action connection TM4 and TM5 domain and active site cycle of thrombin.

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Traumatic hemorrhage is stopped by a hemostatic plug formation, is from a complicated nonlinear and space heterogeneous network proteolytic enzyme biochemical called blood coagulation. We study the spatial dynamic thrombin, the enzyme this network, by developing a fluorogenic substrate to establish methods and space to solve imaging time,thrombin enzyme activity. Clotting stimulation, immobilized tissue factor induced localized clotting enzyme activities of the impulse, in space propagation, have all the characteristics of a tourist excitation wave: constant space velocity, constant amplitude and ignore the initial stimulus once more than activation threshold value. This wave parameters by phospholipid availability or platelet, wave not form in the plasma from type A hemophiliac factors or C lack of viii and XI, the two main mediators condensation positive feedback. Stimulation of the negative feedback of protein C ways and thrombomodulin produce nonstationary model of wave form followed by reduction and destruction. This suggests that the blood can be used as an emotional media, in tourism wave after wave of solidification.

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